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dig gel shift kit 2nd generation  (Roche)


Bioz Manufacturer Symbol Roche manufactures this product  
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    Structured Review

    Roche dig gel shift kit 2nd generation
    Dig Gel Shift Kit 2nd Generation, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dig+gel+shift+kit+2nd+generation/pm37779712-69-13-19
    Average 86 stars, based on 1 article reviews
    dig gel shift kit 2nd generation - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Electrophoretic Mobility Shift Assay:

    Article Title: DNA methylation-independent removable insulator controls chromatin remodeling at the HOXA locus via retinoic acid signaling.
    Article Snippet: .. Electrophoretic mobility shift assay (EMSA) EMSA was performed using the DIG Gel Shift Kit 2nd generation (Roche), according to the manufacturer’s protocol. .. CTCF, RARa, and RXRa proteins were synthesized using coupled in vitro transcription/translation reactions with TNT T7 Quick for PCR (Promega), according to the manufacturer’s protocol.

    Gel Shift:

    Article Title: DNA methylation-independent removable insulator controls chromatin remodeling at the HOXA locus via retinoic acid signaling.
    Article Snippet: .. Electrophoretic mobility shift assay (EMSA) EMSA was performed using the DIG Gel Shift Kit 2nd generation (Roche), according to the manufacturer’s protocol. .. CTCF, RARa, and RXRa proteins were synthesized using coupled in vitro transcription/translation reactions with TNT T7 Quick for PCR (Promega), according to the manufacturer’s protocol.

    Article Title: The Sunflower WRINKLED1 Transcription Factor Regulates Fatty Acid Biosynthesis Genes through an AW Box Binding Sequence with a Particular Base Bias.
    Article Snippet: .. EMSA DNA fragments were labelled with digoxigenin (DIG) following the manufacturer’s instructions (DIG Gel Shift Kit 2nd Generation; Roche Diagnostics, Indianapolis IN, USA), and 30 fmol of labelled DNA was incubated with different amounts of purified HaWRI1_DBD recombinant protein (0–1000 ng) in 20 μL of binding buffer plus 0.05 μg/μL polydIdC. ..

    Article Title: The Brucella abortus two-component system response regulator BvrR binds to three DNA regulatory boxes in the upstream region of omp25 .
    Article Snippet: DNA probes were labeled using the DIG Gel Shift Kit 2nd Generation (Roche®). .. Protein and DNA probes were incubated together following the protocol described in the DIG Gel Shift Kit 2nd Generation (Roche®). ..

    Incubation:

    Article Title: The Sunflower WRINKLED1 Transcription Factor Regulates Fatty Acid Biosynthesis Genes through an AW Box Binding Sequence with a Particular Base Bias.
    Article Snippet: .. EMSA DNA fragments were labelled with digoxigenin (DIG) following the manufacturer’s instructions (DIG Gel Shift Kit 2nd Generation; Roche Diagnostics, Indianapolis IN, USA), and 30 fmol of labelled DNA was incubated with different amounts of purified HaWRI1_DBD recombinant protein (0–1000 ng) in 20 μL of binding buffer plus 0.05 μg/μL polydIdC. ..

    Article Title: The Brucella abortus two-component system response regulator BvrR binds to three DNA regulatory boxes in the upstream region of omp25 .
    Article Snippet: DNA probes were labeled using the DIG Gel Shift Kit 2nd Generation (Roche®). .. Protein and DNA probes were incubated together following the protocol described in the DIG Gel Shift Kit 2nd Generation (Roche®). ..

    Purification:

    Article Title: The Sunflower WRINKLED1 Transcription Factor Regulates Fatty Acid Biosynthesis Genes through an AW Box Binding Sequence with a Particular Base Bias.
    Article Snippet: .. EMSA DNA fragments were labelled with digoxigenin (DIG) following the manufacturer’s instructions (DIG Gel Shift Kit 2nd Generation; Roche Diagnostics, Indianapolis IN, USA), and 30 fmol of labelled DNA was incubated with different amounts of purified HaWRI1_DBD recombinant protein (0–1000 ng) in 20 μL of binding buffer plus 0.05 μg/μL polydIdC. ..

    Recombinant:

    Article Title: The Sunflower WRINKLED1 Transcription Factor Regulates Fatty Acid Biosynthesis Genes through an AW Box Binding Sequence with a Particular Base Bias.
    Article Snippet: .. EMSA DNA fragments were labelled with digoxigenin (DIG) following the manufacturer’s instructions (DIG Gel Shift Kit 2nd Generation; Roche Diagnostics, Indianapolis IN, USA), and 30 fmol of labelled DNA was incubated with different amounts of purified HaWRI1_DBD recombinant protein (0–1000 ng) in 20 μL of binding buffer plus 0.05 μg/μL polydIdC. ..

    Binding Assay:

    Article Title: The Sunflower WRINKLED1 Transcription Factor Regulates Fatty Acid Biosynthesis Genes through an AW Box Binding Sequence with a Particular Base Bias.
    Article Snippet: .. EMSA DNA fragments were labelled with digoxigenin (DIG) following the manufacturer’s instructions (DIG Gel Shift Kit 2nd Generation; Roche Diagnostics, Indianapolis IN, USA), and 30 fmol of labelled DNA was incubated with different amounts of purified HaWRI1_DBD recombinant protein (0–1000 ng) in 20 μL of binding buffer plus 0.05 μg/μL polydIdC. ..



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